Glucagon ELISA

IBL- America is delighted to offer Glucagon ELISA, which may be used in combination with other clinical and laboratory data to aid in diagnosing carbohydrate metabolism disorders related to glucagon levels, including hyper- and hypoglycemia, and pancreatitis.

The Glucagon ELISA is a two-step quantitative capture or ‘sandwich’ type immunoassay. The assay utilizes two highly specific monoclonal antibodies. One glucagon-specific monoclonal antibody is immobilized onto the microplate well to capture the glucagon present in the EDTA plasma sample. The second monoclonal antibody is specific for a second epitope of glucagon and is conjugated to horse radish peroxidase to form the sandwich. The absorbance is measured on a microplate reader at 450 nm. This ELISA can be performed in under two hours utilizing room temperature incubations without shaking the plate. The Glucagon ELISA is 510(k) Exempt - For in vitro diagnostic use only.

The kit contains reagents required to perform the assay, including calibrator stock material, assay controls, strip holder with 12 strips of eight wells, conjugate and TMB substrate. Please read the Glucagon ELISA package insert for full details on the assay. This manual assay may be adapted to open automated analyzers. The user is responsible for validating the performance of this kit, as a manual assay or with any automated analyzer system. 

Thirty-six potential interferents and twenty-one potential cross-reactants were tested in the assay. No cross-reactivity for oxyntomodulin, glicentin, etc. was found.  Please see the package insert for full performance characteristics of the assay. 

Please contact IBL-America with questions or to place your order for the Glucagon ELISA (Glucagon ELISA, IB59142 (IVD) | Human Glucagon Competitive Enzyme-linked Immune-sorbent Assay Kit IBL America).  The catalog number is IB59142.